BioAcyl Corp
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Pavlov, E., Aschar-Sobbi, R., & Campanella, M. (2010). Inorganic Polyphosphate and Energy Metabolism in Mammalian Cells *. Journal of Biological Chemistry, 285(13), 9420–9428. Added by: Dr. Enrique Feoli (17/08/2022, 10:09) Last edited by: Dr. Enrique Feoli (17/08/2022, 10:10) |
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| Resource type: Journal Article DOI: 10.1074/jbc.m109.013011 ID no. (ISBN etc.): 0021-9258, 1083-351X BibTeX citation key: Pavlov2010 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Microenvironment Creators: Aschar-Sobbi, Campanella, Pavlov Collection: Journal of Biological Chemistry |
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| Abstract |
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{textless}p{textgreater}Inorganic polyphosphate (poly P) is a polymer made from as few as 10 to several hundred phosphate molecules linked by phosphoanhydride bonds similar to ATP. Poly P is ubiquitous in all mammalian organisms, where it plays multiple physiological roles. The metabolism of poly P in mammalian organisms is not well understood. We have examined the mechanism of poly P production and the role of this polymer in cell energy metabolism. Poly P levels in mitochondria and intact cells were estimated using a fluorescent molecular probe, 4′,6-diamidino-2-phenylindole. Poly P levels were dependent on the metabolic state of the mitochondria. Poly P levels were increased by substrates of respiration and in turn reduced by mitochondrial inhibitor (rotenone) or an uncoupler (carbonyl cyanide textit{p}-trifluoromethoxyphenylhydrazone). Oligomycin, an inhibitor of mitochondrial ATP-synthase, blocked the production of poly P. Enzymatic depletion of poly P from cells significantly altered the rate of ATP metabolism. We propose the existence of a feedback mechanism where poly P production and cell energy metabolism regulate each other.{textless}/p{textgreater}
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Publisher: Elsevier
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