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Goudswaard, L. J., & Williams, C. M. (2022). Alterations in platelet proteome signature and impaired platelet integrin αiibβ3 activation in patients with covid-19. bioRxiv. Added by: Dr. Enrique Feoli (23/10/2022, 14:03) Last edited by: Dr. Enrique Feoli (23/10/2022, 14:15) |
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| Resource type: Journal Article DOI: 10.1101/2022.10.12.511145 BibTeX citation key: Goudswaard2022 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: COVID-19 Creators: Goudswaard, Williams Collection: bioRxiv |
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| Abstract |
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Background Patients with coronavirus disease-19 (COVID-19) are at increased risk of thrombosis, which is associated with altered platelet function and coagulopathy, contributing to excess mortality.Objectives We aimed to characterise the mechanism of altered platelet function in COVID-19 patients.Methods The platelet proteome, platelet functional responses and platelet-neutrophil aggregates were compared between patients hospitalised with COVID-19 and healthy control subjects using Tandem Mass Tag (TMT) proteomic analysis, Western blotting and flow cytometry.Results COVID-19 patients showed a different profile of platelet protein expression (858 altered out of 5773 quantified). Levels of COVID-19 plasma markers were enhanced in COVID-19 platelets. Gene ontology (GO) pathway analysis demonstrated that levels of granule secretory proteins were raised, whereas some platelet activation proteins, such as the thrombopoietin receptor and PKCα, were lowered. Basally, COVID-19 platelets showed enhanced phosphatidylserine (PS) exposure, with unaltered integrin αIIbβ3 activation and P-selectin expression. Agonist-stimulated integrin αIIbβ3 activation and PS exposure, but not P-selectin expression, were significantly decreased in COVID-19 patients. COVID-19 patients had high levels of platelet-neutrophil aggregates, even under basal conditions, compared to controls. This interaction was disrupted by blocking P-selectin, demonstrating that platelet P-selectin is critical for the interaction.Conclusions Overall, our data suggests the presence of two platelet populations in patients with COVID-19: one with circulating platelets with an altered proteome and reduced functional responses and another with P-selectin expressing neutrophil-associated platelets. Platelet driven thromboinflammation may therefore be one of the key factors enhancing the risk of thrombosis in COVID-19 patients.Essentials- COVID-19 patient platelet function and platelet proteins were compared with healthy controls- Proteomic analysis of platelets indicated that COVID-19 decreased platelet activation proteins- Agonist induced PS exposure and integrin αIIbβ3 activation were impaired in COVID-19- COVID-19 led to maximal levels of P-selectin dependent platelet-neutrophil aggregatesCompeting Interest StatementThe authors have declared no competing interest.
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| Notes |
Red indicates an increase in variable in patients with COVID- 19 vs healthy controls and blue indicates a decrease. Added by: Dr. Enrique Feoli Last edited by: Dr. Enrique Feoli |