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Mousa, S. A., Machelska, H., & Schäfer, M. (2002). Immunohistochemical localization of endomorphin-1 and endomorphin-2 in immune cells and spinal cord in a model of inflammatory pain. Journal of Neuroimmunology, 126(1), 5–15. Added by: Dr. Enrique Feoli (14/10/2023, 16:00) Last edited by: Dr. Enrique Feoli (14/10/2023, 16:01) |
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| Resource type: Journal Article DOI: https://doi.org/10.1016/S0165-5728(02)00049-8 ID no. (ISBN etc.): 0165-5728 BibTeX citation key: Mousa2002 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Analgesia Creators: Machelska, Mousa, Schäfer Collection: Journal of Neuroimmunology |
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| Abstract |
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Recently, two novel highly selective μ-opioid receptor (MOR) agonists, endomorphin-1 and endomorphin-2, have been isolated from bovine as well as human brains and were proposed to be the endogenous ligand for MOR. Later, endomorphin-1 and endomorphin-2 have been detected in the immune system of rats and humans using radioimmunoassay in combination with reverse-high-phase-liquid chromatography. In the present study, we analyzed the expression of endomorphin-1, endomorphin-2 and MOR by immunohistochemistry in a model of Freund's complete adjuvant (FCA)-induced painful inflammation. While MOR was upregulated on peripheral and central nerve terminals, inflammation did not alter endomorphin-2 expression in nerve fibers either in the dorsal horn of the spinal cord or in subcutaneous tissue. Endomorphin-1 and endomorphin-2 were expressed in immune cells (macrophage/monocytes) in the medullary region of the popliteal lymph nodes. The proportion of immunocytes (macrophage/monocytes, lymphocytes) containing endomorphin-1 and endomorphin-2 was increased in inflamed lymph nodes and subcutaneous paw tissue of animals with local inflammatory pain. Taken together, the upregulation of MOR and of its endogenous ligands endomorphin-1 and endomorphin-2 in immunocytes suggests an involvement of these opioid peptides in the peripheral control of inflammatory pain.
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