BioAcyl Corp
BioAcyl Corp |
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Ytting, C. K., Fuglsang, A. T., Kalervo Hiltunen, J., Kastaniotis, A. J., Özalp, V. C., & Nielsen, L. J., et al. (2012). Measurements of intracellular ATP provide new insight into the regulation of glycolysis in the yeast Saccharomyces cerevisiae. Integrative Biology, 4(1), 99–107. Added by: Dr. Enrique Feoli (20/11/2020, 19:14) Last edited by: Dr. Enrique Feoli (20/11/2020, 19:14) |
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| Resource type: Journal Article DOI: 10.1039/c1ib00108f ID no. (ISBN etc.): 1757-9708 BibTeX citation key: Ytting2012 View all bibliographic details  |
Categories: BioAcyl Corp Subcategories: Adenylate energy system Creators: Fuglsang, Kalervo Hiltunen, Kastaniotis, Nielsen, Olsen, Özalp, Ytting Collection: Integrative Biology |
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| Abstract |
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Glycolysis in the yeast Saccharomyces cerevisiae exhibits temporal oscillation under anaerobic or semianaerobic conditions. Previous evidence indicated that at least two membrane-bound ATPases, the mitochondrial F0F1ATPase and the plasma membrane P-type ATPase (Pma1p), were important in regulating the glycolytic oscillation. Measurements of intracellularATP provide a unique tool to understand the role of these membrane ATPases and how their activities are regulated. We have constructed a new nanobiosensor that can perform time-resolved measurements of intracellularATP in intact cells. Measurements of the temporal behaviour of intracellularATP in a yeast strain with oscillating glycolysis showed that, in addition to oscillation in intracellularATP, there is an overall slow decrease in intracellularATP because the ATP consumption rate exceeds the ATP production in glycolysis. Measurements of the temporal behaviour of intracellularATP in yeast strains lacking either of the two membrane bound ATPases have confirmed that F0F1ATPase and Pma1p contribute significantly to the ATP consumption in the cell and to the regulation of glycolytic oscillation. Furthermore, our measurements also demonstrate that ATPase activity is under strict control. In the absence of glucoseATPase activity is switched off, and the intracellularATP concentration is high. When glucose is added to the cells the ATP concentration starts to decrease, because ATP consumption exceeds ATP production by glycolysis. Finally, when glucose is used up, the ATP consumption stops immediately. Thus, glucose or some compound derived from glucose must be involved in controlling the activity of these two ATPases.
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